Analysis Note

ControlLung tissue, mouse brain lysate.

Application

Anti-RAGE Antibody, clone DD/A11 or A11 is an antibody against RAGE for use in ELISA, IC, IH & WB.

Immunohistochemistry:
A previous lot of this antibody was shown to be reactive on paraffin embedded tissue sections.

Immunocytochemistry:
A previous lot was shown to work on cells fixed with ice cold acetone using a Cy3 conjugated secondary antibody.

ELISA:
A previous lot was shown to work on direct ELISA.

Immunoblotting:
Recognizes a band of ~48kDa in bovine lung extract.

Optimal working dilutions must be determined by end user.

General description

RAGE (Receptor for Advanced Glycosylation End Products) is a 35 kDa cell surface receptor that binds molecules modified by advanced glycation end products (AGEs), an irreversible non-enzymatic reaction occuring from the interaction of proteins and lipids with glucose. The increased expression of RAGE is associated with many disorders, such as diabetic vasculopathy, neuropathy, retinopathy and neuropathy, Alzheimer's disease and immune/inflammatory reactions of the vessel walls. AGE/RAGE accumulates at sites of vascular disease in diabetes, but blockade of RAGE suppresses development of atherosclerosis and vascular disorders. RAGE binds to amyloid beta, which is overproduced in neurons and vessels in Alzheimer′s disease, and this interaction results in oxidative stress leading to neuronal degeneration.

Immunogen

Recombinant truncated extracellular part of RAGE produced in E. coli.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified mouse monoclonal IgG2a in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, and 0.1% sodium azide.

Quality

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot:
1:500 dilution of this lot detected RAGE on 10 µg of Mouse Brain lysates.

Specificity

RAGE (Receptor for Advanced Glycosylated End Products). The antibody recognizes both natural and recombinant RAGE.

Target description

48 kDa